Ruprecht-Karls-Universität Heidelberg
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Plant Molecular Biology

Dr. Eric Linster

Dr. Eric Linster
Dr. Eric Linster
Im Neuenheimer Feld 360
69120 Heidelberg
Fon +49 6221 54-5603
Fax +49 6221 54-5859
ed.grebledieh-inu.soc TEA retsnil.cire

 


Nα-terminal acetylation (NTA) is one of the most common protein modifications in eukaryotes, affecting up to 80% of human cytosolic proteins. Despite the high abundance of NTA, the biological significance is still enigmatic. In yeast NTA alters the turnover of a subset of proteins by generating a N-terminal degradation signal. NTA occurs in a cotranslational manner and is catalysed by Nα-terminal acetyltransferase (Nat), which are composed of a catalytic and an auxiliary subunit. In yeast and human five Nat´s (NatA, NatB, NatC, NatD and NatE) were identified that differ in their substrate specificity. Database analyses reveal that A. thaliana contains orthologues for all subunits of these Nats. Isolation of T-DNA insertion lines that constitute loss-of-function mutants for both subunits of NatA show an arrest at the globular stage of embryo development. This points to significant functions of NTA during embryogenesis in A. thaliana. An artificial microRNA approach allows to specifically down-regulate the catalytic subunit of NatA to overcome the embryo-lethal phenotype and to investigate further functions of NTA during vegetative and generative growth phase. In addition to its Nat activity the catalytic subunit of the NatE complex shows also lysine Nε-acetyltransferase activity.
 

- kindly supported by Schmeil-Stiftung, Heidelberg -

/var/www/cos/ / http://www.cos.uni-heidelberg.de/ Dr. Eric Linster _e