Current Research

We study the cellular and molecular processes underlying the formation of the nervous system. In particular, we are interested in interactions of growing axons / growth cones with their environment and the transformation of such interactions into directed growth.

Using various in vitro, in vivo and in ovo systems of the chick embryo retina, we investigate the potential role of cell adhesion molecules (CAMs) for growth and orientation of retinal ganglion cell axons.  We also study the role of the cytoskeletal structures in the growth cone, characterising known and novel components. In addition, the signalling components mediating between cell membrane and growth cone cytoskeleton are investigated.

For our studies we make use of biochemical, cell biological and moleculer biological assays such as laser inactivation (CALI), cellular displays, yeast-2-hybrid assays, knock down (siRNA), overexpression, in ovo electroporation, viral transfection and nucleofection etc..

Future Projects and Goals

Cell adhesion molecules, cytoskeletal components and intracellular signalling components will be investigated for their role in axonal orientation. In particular we want to study their functions in organotypic / histotypic environment and in vivo, which is ideal in the avian system (in ovo). In addition, we also investigate the retina of transgenic mouse embryos mutated in the genes of our interest. Our aim is to contribute to a better understanding of the „wiring of the brain“ during higher vertebrate embryogenesis.