Im Neuenheimer Feld 230
Fon +49 6221 54-5637
ed.grebledieh-inu.soc TEA regeoh.einafets
I’m interested in transcriptional processes and the role of canonical Wnt signaling during regeneration in the freshwater polyp Hydra magnipapillata. Regeneration is directly connected to stem cell research through self-renewal and the recruitment of stem cells during regeneration processes. Previous work has shown that beta-Catenin dependent Wnt-Signaling is linked to stem cell proliferation. The molecular basis of this process, especially on a transcriptional and protein level, is largely unknown to date. Therefore the principal aim of my PhD project is to perform an analysis of the early transcriptional response to the regeneration stimulus in Hydra via RNA-Seq. I’m trying to investigate transcriptional control mechanisms of Wnt signaling by a dissection of the cis-regulatory interplay between canonical and non-canonical Wnt signaling (Wnt-3 and -11 and Wnt-5 and -8, respectively) during the regeneration process.
The project also includes epigenetic analyses of transcriptional regulators in beta-Catenin activated cells by ChIP-Seq. Proteins of interest for ChIP-analysis are beta-Catenin, Sox1B transcription factors, and proteins of the PRC2 complex. This work will elucidate mechanisms of molecular cell fate determination and transcriptional profiles of epithelial stem cells involved in the regeneration response.