Ruprecht-Karls-Universität Heidelberg
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Molekulare Biologie der Pflanzen

Dr. Hannah Birke

Dr. Hannah Birke
hannah.birke AET

Evolution of Cysteine Synthesis in Phototrophic Organisms
Cysteine is the pivotal source of reduced sulfur for all organic compounds. In higher plants it is produced in a two step reaction from serine, acetyl-CoA, and sulfide by the enzymes serine acetyltransferase (SAT) and O-acetylserine(thiol)lyase (OAS-TL). SAT and OAS-TL form the so-called cysteine synthase complex (CSC). Remarkably, the function of complex formation is not substrate channeling but constitutes the basis for demand-driven regulation of cysteine synthesis. Additionally, SAT and OAS-TL in cytosol, plastids, and mitochondria contribute to a different extend to total cellular cysteine production. The regulatory function of complex formation and functional compartmentation of cysteine synthesis in higher plants seem to facilitate a delicate but efficient mechanism to adjust cysteine production to the demands of the cell and subcellular compartments The significance of both CSC formation and compartmentation in higher plants is addressed by an evolutionary approach.!
 To this end, conservation of SAT and OAS-TL protein structures as well as compostion of the CSC is anylyzed in Arabidopsisthaliana and Glycine max in comparison to eubacterial and cyanobacterial proteins. Evolutionary conservatin of compartmentation is investigated using the moss Physcomitrella patens as a representative of early land plants. Finally, a special focus concentrates on the function of compartmentation in A. Thaliana and the significance of sulfide consumption by cysteine production in a specific subcellular compartment. In this context, effects of excess sulfide on sulfur metabolism are analyzed in wild type plants and plants lacking cysteine production in specific subcellular compartments.

gefördert durch die Schmeil Stiftung, Heidelberg

/var/www/cos/ / Dr. Hannah Birke