Dr. Markus Kiefer
Dr. Markus Kiefer
Im Neuenheimer Feld 345, Zi. R224
Fon +49 6221 54-4679
Fax +49 6221 54-5508
markus.kiefer AET cos.uni-heidelberg.de
Management of technology platforms - AG Koch
Bioinformatics and biodiversity informatics
Since the department of biodiversity and plant systematics has considerable computational resources at its disposal we are able to do most sequence analysis tasks in-house within a reasonable timeframe. The tools are available on local server machines, so they are customizable if needed. Several webbased databases do exist as information collections for genera of interest for research projects. Phylogenetic inference is done routinely using all major algorithms and criteria (distance-based methods as well as heuristic searches, multi-parallel bayesian analysis).
DNA based analyses
The department's equipment allows to carry out most modern protocols ranging from PCR based methods to cloning of DNA fragments in an S1 environment. Two capillary sequencers (Amersham MegaBace 500 and 1000) provide the opportunity for high through-put sequence and fragment analysis and efficient processing of raw data. Currently we routinely perform AFLP and microsatellite analysis. The facilities also allow the offer of regular courses for students in molecular biology (E1-practical course) in an additional students lab.
Flow cytometry, chromosome counts
Flow cytometry is done using a Partec CyFlow space cytometer fitted with UV lighting and a green laser for propidium iodide and DAPI detection. This allows for high throughput ploidy analysis and C-value determination. Additionally chromosome preparation and counting by means of epifluorescence microscopy are well established.