Prof. Dr. Sabine Strahl
Prof. Dr. Sabine Strahl
Im Neuenheimer Feld 360
Fon +49 6221 54-6286
Fax +49 6221 54-5859
sabine.strahl AET cos.uni-heidelberg.de
Office hours Monday 1:15- 2:00 pm
The Strahl Lab - Glycobiology: Sweet Science
Glycosylation is one of the most abundant and complex protein modifications known. In many biological systems more than half of all proteins are glycosylated and glycan chains are highly crucial for a wide range of biological processes. Our laboratory is interested in various aspects of glycobiology in eukaryotic organisms with a main focus on O-mannosylation, an evolutionarily conserved, essential protein modification with immense impact on a variety of cellular processes in humans. Defective O-mannosylation results in severe muscular dystrophy and malfunctions of the brain and the eyes. Having identified the first protein O-mannosyltransferase PMT1 in the model organism yeast, our work has substantially contributed to the understanding of protein O-mannosylation in eukaryotes. The laboratory continues to work on the molecular organization of the O-mannosylation machinery in the endoplasmic reticulum (ER) and the role of O-mannosyl glycans for growth and development of fungi and mammals.
Protein O-Mannosylation, an essential modification that is conserved from yeast to human
O-mannosyl glycans are short linear oligo-saccharides linked via an alpha-glycosidically-bound mannose to Ser and Thr residues. Biosynthesis is initiated at the endoplasmic reticulum (ER) by the transfer of mannose from dolichyl phosphate-activated mannose to Ser or Thr residues of proteins that are entering the secretory pathway. Further chain elongation takes place in the Golgi apparatus using nucleotide-activated sugars as donors.
The initial mannosyltransfer reaction is catalyzed by an essential family of Dol-P-mannose: protein O-mannosyl-transferases (PMTs) that is evolutionarily conserved from yeast to humans. Focusing on PMTs we aim to understand how the ER O-mannosylation machinery works. Further, we analyze the occurrence and functions of O-mannosyl glycans in the eukaryotic model system baker's yeast and in mammals.
The biosynthetic pathway of O-mannosyl glycans in yeast (left) and mammals (right).
Please click on image to enlarge
Find out more about our recent projects >>>>
Castells-Ballester J, Ewa Zatorska E, Meurer M, Neubert P, Metschies A, Knop M and Strahl S. (2018). Monitoring Protein Dynamics in Protein-Mannosyltransferase Mutants In Vivo by Tandem Fluorescent Protein Timers. Molecules. 23(10), 2622; https://doi.org/10.3390/molecules23102622
Zatorska E, Gal L, Schmitt J, Bausewein D, Schuldiner M and Strahl S. (2018). Cellular Consequences of Diminished Protein O-Mannosyltransferase Activity in Baker’s Yeast. Int. J. Mol. Sci. 18, 1226; doi:10.3390/ijms18061226