Prof. Karin SchumacherCell Biology
Due to their sessile lifestyle, plants need to efficiently adapt their metabolic and developmental program to changing and often unfavourable environmental conditions. Adaptation often involves considerable fluctuations of metabolite and ion concentrations between tissues, cells and organelles that are mediated either by membrane transport or vesicular trafficking. Our lab has shown that in the model plant Arabidopis the V-ATPase, a highly conserved eukaryotic proton-pump, does not only fuel secondary active transport processes but also plays an important role in the regulation of endocytic and secretory trafficking.
Current research aims at understanding the regulatory networks that control V-ATPase activity as well as the identification of the biological interactions that depend on the activity of this complex proton-pump. Using Arabidopsis as our main model system, we employ genetics and cell biology as well as biochemistry and physiology to provide knowledge that could eventually lead to novel strategies for improved crop yield under stress conditions.
The group also has a strong structural emphasis (see also Electron Microscope Facility) having state-of-the-art equipment for antigen localization at the electron microscopic (cryosectioning, high pressure freezing) and light microscopic (CLSM) levels.
Anne Kriegel, Zaida Andrés, Anna Medzihradszky, Falco Krüger, Stefan Scholl, Simon Delang, M. Görkem Patir-Nebioglu, Gezahegn Gute, Haibing Yang, Angus S. Murphy, Wendy Ann Peer, Anne Pfeiffer, Melanie Krebs, Jan U. Lohmann and Karin Schumacher (2015) Job Sharing in the Endomembrane System: Vacuolar Acidification Requires the Combined Activity of V-ATPase and V-PPase. Plant Cell, doi: 10.1105/tpc.15.00733
Yu Luo, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer G. Irani, Simone Di Rubbo, Lutz Neumetzler, Praveen Krishnamoorthy, Isabelle Van Houtte, Evelien Mylle, Volker Bischoff, Samantha Vernhettes, Johan Winne, Jirí Friml, York-Dieter Stierhof, Karin Schumacher, Staffan Persson & Eugenia Russinova (2015) V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. Nature Plants 1, Article number: 15094, doi:10.1038/nplants.2015.94
Keinath NF, Waadt R, Brugman R, Schroeder JI, Grossmann G, Schumacher K, Krebs M. (2015) Live Cell Imaging with R-GECO1 Sheds Light on flg22- and Chitin-Induced Transient [Ca2+]cyt Patterns in Arabidopsis. Mol Plant. in press.
Schumacher K. (2014). pH in the plant endomembrane system - an import and export business. Curr Opin Plant Biol 22C:71–76.
Viotti C, Kruger F, Krebs M, Neubert C, Fink F, Lupanga U, David Scheuring, Boutté Y, Frescatada-Rosa M, Wolfenstetter S, Sauer N, Hillmer S, Grebe M, Schumacher K. (2013). The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis. Plant Cell 25:3434–3449.
Krebs M, Schumacher K. (2013). Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots. Cold Spring Harb Protoc 8:776-780.
Krebs M, Held K, Binder A, Hashimoto K, Herder Den G, Parniske M, Kudla J, Schumacher K. (2012). FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca2+ dynamics. Plant Journal 69:181-192.
Scheuring D, Viotti C, Krüger F, Künzl F, Sturm S, Bubeck J, Hillmer S, Frigerio L, Robinson DG, Pimpl P, Schumacher K. (2011) Multivesicular bodies mature from the trans-Golgi network/early endosome in Arabidopsis. Plant Cell 23:3463–3481.
Drakakaki G, van de Ven W, Pan S, Miao Y, Wang J, Keinath NK, Weatherly B, Jiang L, Schumacher K, Hicks G, Raikhel N. (2011). Isolation and proteomic analysis of the SYP61 compartment reveal its role in exocytic trafficking in Arabidopsis. Cell Res 1–12.
Schumacher K, Krebs M. (2010). The V-ATPase: small cargo, large effects. Curr Opin Plant Biol 13:724–730.
Viotti C, Bubeck J, Stierhof Y-D, Krebs M, Langhans M, van den Berg W, van Dongen W, Richter S, Geldner N, Takano J, Jürgens G, de Vries SC, Robinson DG, Schumacher K. (2010). Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle. Plant Cell 22:1344–1357.
Krebs M, Beyhl D, Görlich E, Al-Rasheid KAS, Marten I, Stierhof Y-D, Hedrich R, Schumacher K. (2010). Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation. Proc Natl Acad Sci USA 107:3251–3256.