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Pereira LabPublications

Kanamaru, T., Neuner, A., Kurtulmus, B. and Pereira, G. (2021). Balancing the length of distal tip is key for stability and signalling function of primary cilia. BioRxiv, https://doi.org/10.1101/2021.06.04.447095.


Fueller, J., Meurer, M., Herbst, K., Gubicza, K., Kurtulmus, B., Knopf, J.D., Kirrmaier, D., Buchmuller, B., Pereira, G., Lemberg, M.K. and Knop, M. (2020). CRISPR/Cas12a-assisted PCR tagging of mammalian genes. J Cell Biol. 219 (6): e201910210 (BioRxiv 473876).


Viol, L., Hata, S., Pastor-Peidro, A., Neuner, A., Murke, F., Wuchter, P., Ho, A.D., Giebel, B., and Pereira, G. (2020). Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol. 219, 10.1083/jcb.201907136.


Hata, S., Peidro, A.P., Panic, M., Liu, P., Atorino, E., Funaya, C., Jäkle, U., Pereira, G., and Schiebel, E. (2019). The balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly. Nat. Cell Biol. 21, 1138–1151.


Kurtulmus, B., Yuan, C., Schuy, J., Neuner, A., Hata, S., Kalamakis, G.,  Martin-Villalba, A. and Pereira, G. (2018). LRRC45 contributes to early steps of axoneme extension. J. Cell Sci., 131, jcs223594.


Martins, L.R., Bung, R.K., Koch, S., Richter, K., Schwarzmuller, L., Terhardt, D., Kurtulmus, B., Niehrs, C., Rouhi, A., Lohmann, I., et al. (2018). Stk33 is required for spermatid differentiation and male fertility in mice. Dev Biol 433, 84–93.


Uddin, B., Partscht, P., Chen, N., Neuner, A., Weiß, M., Hardt, R., Jafarpour, A., Heßling, B., Ruppert, T., Lorenz, H., et al. (2018). The human phosphatase CDC14A modulates primary cilium length by regulating centrosomal actin nucleation. EMBO Rep. 20, e46544.


Maekawa H, Neuner A, Rüthnick D, Schiebel E, Pereira G, Kaneko Y. (2017). Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha. Elife. 10.7554/eLife.24340


Caydasi, A.K., Khmelinskii, A., Duenas-Sanchez, R., Kurtulmus, B., Knop, M. and Pereira, G. (2017). Temporal and compartment-specific signals co-ordinate mitotic exit with spindle position. Nat. Comm., doi:10.1038/ncomms14129.


Meitinger, F. and Pereira, G. (2017). The septin-associated kinase Gin4 recruits Gps1 to the bud neck for spatial control of Rho1 and Cdc42. Mol. Biol. Cell, 28:883-889.


Gryaznova, Y., Caydasi, A.K., Malengo, G., Sourjik, V. and Pereira G. (2016). A FRET-based study reveals site-specific regulation of spindle position checkpoint proteins at yeast centrosomes. eLife. published ahead of print (eLife 2016;10.7554/eLife.14029).


Kurtulmus B, Wang W, Ruppert T, Neuner A, Cerikan B, Viol L, Dueñas-Sánchez R, Gruss OJ, Pereira G. (2016). WDR8 is a centriolar satellite and centriole-associated protein that promotes ciliary vesicle docking during ciliogenesis. J Cell Sci. 129(3):621-36.


Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, Kurtulmus B, Novikov O, Spatz J, Pereira G, Hübner W, Brodski C, Tucker KL, Blaess S. (2016). Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 409(1):55-71.


Meitinger, F., Khmelinskii, A., Morlot, S., Kurtulmus, B., Palani, S., Andres-Pons, A., Hub, B., Knop, M., Charvin, G. and Pereira, G. (2014). A memory system of negative polarity cues prevents replicative aging. Cell, 159: 1056-1069.

Comment in: Wedlich-Söldner, R. (2014). A longer life for yeast with good memory. Dev. Cell, 31:391-392.


Caydasi, A.K.*, Micoogullari, Y*, Kurtulmus, B., Palani. S. and Pereira G. (2014). The 14-3-3 protein Bmh1 functions in the spindle position checkpoint by breaking Bfa1 asymmetry at yeast centrosomes. Mol. Biol. Cell, mbc.E14-04-0890 (epub ahead of print). * Co-first authors.


Klinger, M., Wang W., Kuhns, S., Baerenz, F., Draeger-Meurer, S., Pereira , G.* and Gruss, O.J.* (2014). The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone. Mol. Biol. Cell, 25:495-507.


Lombardi, I.M.*, Palani, S.*, Meitinger, F., Darieva, Z., Hofmann, A., Sharrocks, A. and Pereira, G. (2013). Lre1 direclty inhibits the NDR/Lats kinase Cbk1 at the cell division site in a phosphorylation dependent manner. Curr. Biol., 23:1736-1745. * Co-first authors.


Meitinger, F., Palani, S., Hub, B. and Pereira, G. (2013). Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol. Biol. Cell, 24:1290-1304.


Kuhns,  S., Schmidt, K.N., Reymann, J., Gilbert, D.F., Neuner, A., Hub, B., Carvalho, R., Wiedemann, P., Zentgraf, H., Erfle, H., Klingmüller, U., Boutros, M. and Pereira, G. (2013). The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis. J. Cell Biol.,  doi: 10.1083/jcb.201206013.


Schmidt, K.N., Kuhns, S., Neuner, A., Hub, B., Zentgraf, H. and Pereira, G. (2012). Cep164 mediates  vesicular docking to the mother centriole during early steps of ciliogenesis. J.Cell Biol., 199:1083-1101.


Khmelinskii, A., P.J. Keller, A. Bartosik, M. Meurer, J.D. Barry, B.R. Mardin, A. Kaufmann, S. Trautmann, M. Wachsmuth, G. Pereira, W. Huber, E. Schiebel and M. Knop (2012). Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat. Biotechnol., 30:708-714.

Caydasi, A.K.*, Lohel, M.*, Grünert, G., Dittrich, P., Pereira, G.** and Ibrahim, B.** (2012). A dynamical model of the spindle position checkpoint. Mol. Syst. Biol., 8:582.
*  Co-first authors
** Corresponding authors

Palani, S., Meitinger, F., Boehm, M.E., Lehmann, W.D., and Pereira, G. (2012). Cdc14-dependent dephosphorylation of Inn1 contributes to Inn1-Cyk3 complex formation. J. Cell Sci., Mar 27 (Epub ahead of print).

Bertazzi, D.L., Kurtulmus, B. and Pereira, G. (2011). The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4. J. Cell Biol., 193:1033-1048.

Meitinger, F., Boehm, M.E., Hofmann, A., Hub, B., Zentgraf, H., Lehmann, W.D. and Pereira, G. (2011). Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes & Dev., 25:875-888.

Steinbrück, L., Pereira, G. and Efferth, T. (2010). Effects of artesunate on cytokinesis and G2/M cell cycle progression of tumour cells and budding yeast. Cancer Genomics & Proteomics, 7:337-346.

Caydasi, A.K., Kurtulmus, B. , Orrico, M.I.L. , Hofmann, A. , Ibrahim, B. and Pereira, G. (2010). Elm1 kinase activates the spindle position checkpoint kinase Kin4. J. Cell Biol., 190(6):975-989.
Comment in: Leslie , M. (2010). Elm1 sparks the SPOC. J. Cell Biol., 190 (6): 944.

Meitinger, F., Petrova, B., Lombardi, I.M., Bertazzi, D.L., Hub, B. Zentgraf H. and Pereira, G. (2010). Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic exit network regulates cytokinesis in budding yeast. J. Cell Sci., 123: 1851-1861.
Comment in: Bradbury, J. (2010). Mitotic-exit networks with cytokinesis. J. Cell Sci. 123, 1103e.

Caydasi,A.K. and Pereira, G. (2009). Spindle alignment regulates the dynamic association of checkpoint proteins with yeast spindle pole bodies. Dev Cell. Jan;16(1):146-56.

Sanchez-Diaz, A., V. Marchesi, S. Murray, R. Jones, G. Pereira, R. Edmondson, T. Allen and K. Labib (2008). Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol.,10: 395-406.

Maekawa, H., C. Priest, J. Lechner, G. Pereira and E. Schiebel (2007). The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J. Cell. Biol., 179: 423-436.

Pereira G. and E. Schiebel (2005). Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol. Cell, 19:209-221.
Comment in: Yoshida S., M. Guillet and D. Pellman (2005). MEN signaling: daughter bound pole must escape her mother to be fully active. Dev. Cell, 9:168-170.

Fischer T., S. Rodríguez-Navarro, G. Pereira, A. Rácz, E. Schiebel and E. Hurt (2004). Yeast centrin Cdc31 functions in nuclear mRNA export. Nat. Cell Biol., 6:840-848.

Pereira G. and E. Schiebel (2003). Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science, 302:2120-2124.
Comment in: Higuchi T. and F. Uhlmann (2003). Cell cycle: passenger acrobatics. Nature, 426:780-781.

Usui T., H. Maekawa, G. Pereira and E. Schiebel (2003). The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. EMBO J., 22:4779-4793.

Pereira G., C. Manson, J., Grindlay and E. Schiebel (2002). Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. J. Cell Biol., 157:367-379.
Comment in: Wells W.A. (2002). Cdc14p turns MEN on and off. J. Cell Biol., 157:345.

Tanaka T.U., N. Rachidi, C. Janke, G. Pereira, M. Galova, E. Schiebel, M. J. R. Stark and K. Nasmyth (2002). The Ipl1/Sli15 (Aurora B/INCENP) kinase complex promotes re-orientation of kinetochore-spindle pole connection and ensures its bi-orientation of sister chromatids in yeast. Cell, 108:317-329.

Pereira G., T. Tanaka, K. Nasmyth and E. Schiebel (2001). Defined modes of spindle pole bodies inheritance and segregation of the checkpoint protein Bfa1p in yeast. EMBO J., 20:6359-6370.
Comment in: Bornens M. and M. Piel (2002). Centrosome inheritance: birthright or the privilege of maturity? Curr Biol., 12:R71-3.

Pereira G., T. Höfken, J. Grindlay, C. Manson and E. Schiebel (2000). The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol. Cell, 6:1-10.
Comment in: Hoyt M.A. (2000). Exit from mitosis: spindle pole power. Cell, 102:267-270.

Pereira G., U. Grueneberg, M. Knop and E. Schiebel. (1999) Interaction of the yeast gamma-tubulin complex binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy. EMBO J., 18:4180-4196.

Knop M., K. Siegers, G. Pereira, W. Zachariae, B. Winsor, K. Nasmyth and E. Schiebel (1999). Epitope tagging of yeast genes using a PCR-base strategy: more tags and improved practical routines. Yeast, 15:963-972.

Pereira G., M. Knop, and E. Schiebel (1998). Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell-cycle dependent phosphorylation on the nuclear side of the spindle pole body. Mol. Biol. Cell, 9:775-793.

Knop M., G. Pereira, S. Geissler, K. Grein, and E. Schiebel (1997). The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. EMBO J., 16:1550-1564.


Assis A.I., Pereira, G., Azzolini, A. and Barbosa, J.E. (1991). Effect of the in vitro ageing on the restriction of sheep erythrocyte lysis by horse serum. Scand J Immunol., 33:579-84.





Review articles


Caydasi, A.K. and Pereira, G. (2016). Evaluation of the dynamicity of mitotic exit network and spindle position checkpoint components on spindle pole bodies by fluorescence recovery after photobleaching (FRAP). Methods in Molecular Biology, in press.


Meitinger, F. and Pereira, G. (2016). Visualization of cytokinesis events in budding yeast by transmission electron microscopy. Methods in Molecular Biology, in press.


Meitinger, F., Palani, S. and Pereira, G. (2016). Detection of the phosphorylation status of cytokinesis components. Methods in Molecular Biology, in press.


Caydasi, A.K. and Pereira, G. (2012). SPOC alert - when chromosomes get the wrong direction. Exp. Cell Res., in press.

Meitinger, F., Palani, S. and Pereira, G. (2012). The power of MEN in cytokinesis. Cell Cycle, 11:219-228.

Pereira, G. and Yamashita, Y. (2011). Fly meets yeast: checking correct orientation of cell division. Trends Cell Biol., 21:526-533.

Caydasi, A.K., Ibrahim, B. and Pereira, G. (2010). Monitoring spindle orientation: spindle position checkpoint in charge. Cell Div., 5:28.

Pereira G. and E. Schiebel (2004). Cdc14 phosphatase resolves the rDNA segregation delay. Nat. Cell Biol., 6:473-475.

Pereira G. and E. Schiebel (2001). The role of the yeast spindle pole body and mammalian centrosome in regulation of late mitotic events. Curr. Opin. Cell Biol., 13:762-769.

Knop M., G. Pereira and E. Schiebel (1999). Microtubule organization by the budding yeast spindle pole body. Biol. Cell, 91:291-304.

Pereira G. and E. Schiebel (1997). Centrosome microtubule nucleation. J. Cell Sci., 110:295-300.